人B淋巴细胞GM12878
BLUEFBIO™ Product Sheet
细胞名称 |
人B淋巴细胞GM12878 |
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货物编码 |
BFN60810358 |
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产品规格 |
T25培养瓶x1 |
1.5ml冻存管x2 |
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细胞数量 |
1x10^6 |
1x10^6 |
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保存温度 |
37℃ |
-198℃ |
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运输方式 |
常温保温运输 |
干冰运输 |
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安全等级 |
1 |
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用途限制 |
仅供科研 3类 |
培养体系 |
90%DMEM(不含丙酮酸钠)+10%FBS+1%三抗 |
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培养温度 |
37℃ |
二氧化碳浓度 |
5% |
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简介 |
人B淋巴细胞GM12878取自女性外周血。 |
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注释 |
Part of: CEPH/Utah pedigree cell line collection. Part of: ENCODE project common cell types; tier 1. Part of: Genetic Testing Reference Material (GeT-RM) samples. Part of: Genome in a Bottle (GIAB) consortium samples. Part of: International Genome Sample Resource (1000 genomes project) cell lines. Registration: CEPH Families Reference Panel; 146302. Registration: National Institute of Standards and Technology, Standard Reference Materials; HG001. Population: Utah residents with ancestry from Northern and Western Europe. Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV). Omics: CNV analysis. Omics: Deep exome analysis. Omics: Deep proteome analysis. Omics: DNA methylation analysis. Omics: Deep RNAseq analysis. Omics: Genome sequenced. Omics: H3K27ac ChIP-seq epigenome analysis. Omics: H3K27me3 ChIP-seq epigenome analysis. Omics: H3K36me3 ChIP-seq epigenome analysis. Omics: H3K4me1 ChIP-seq epigenome analysis. Omics: H3K4me2 ChIP-seq epigenome analysis. Omics: H3K4me3 ChIP-seq epigenome analysis. Omics: H3K79me2 ChIP-seq epigenome analysis. Omics: H3K9ac ChIP-seq epigenome analysis. Omics: H3K9me3 ChIP-seq epigenome analysis. Omics: H4K20me1 ChIP-seq epigenome analysis. Omics: Mitochondrial genome sequenced. Omics: SNP array analysis. Omics: Transcriptome analysis. Derived from sampling site: Peripheral blood. |
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基因突变 |
CYP2C19 p.Pro227Pro (c.681G>A); cryptic splice acceptor activation; allele CYP2C19*2 (Coriell). |
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HLA信息 |
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STR信息 |
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参考文献 |
PubMed=21418647; DOI=10.1186/gb-2011-12-3-r25 Morcos L., Ge B., Koka V., Lam K.C., Pokholok D.K., Gunderson K.L., Montpetit A., Verlaan D.J., Pastinen T. Genome-wide assessment of imprinted expression in human cells. Genome Biol. 12:R25.1-R25.14(2011)
PubMed=23325432; DOI=10.1101/gr.147942.112 Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M. Dynamic DNA methylation across diverse human cell lines and tissues. Genome Res. 23:555-567(2013)
PubMed=23676674; DOI=10.1038/nature12223 Wu L., Candille S.I., Choi Y., Xie D., Jiang L., Li-Pook-Than J., Tang H., Snyder M. Variation and genetic control of protein abundance in humans. Nature 499:79-82(2013)
PubMed=24185094; DOI=10.1038/nbt.2728 Selvaraj S., Dixon J.R., Bansal V., Ren B. Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing. Nat. Biotechnol. 31:1111-1118(2013)
PubMed=26621101; DOI=10.1016/j.jmoldx.2015.08.005 Pratt V.M., Everts R.E., Aggarwal P., Beyer B.N., Broeckel U., Epstein-Baak R., Hujsak P., Kornreich R., Liao J., Lorier R., Scott S.A., Smith C.H., Toji L.H., Turner A., Kalman L.V. Characterization of 137 genomic DNA reference materials for 28 pharmacogenetic genes: a GeT-RM collaborative project. J. Mol. Diagn. 18:109-123(2016)
PubMed=30485824; DOI=10.1016/j.celrep.2018.10.096 Papp E., Hallberg D., Konecny G.E., Bruhm D.C., Adleff V., Noe M., Kagiampakis I., Palsgrove D., Conklin D., Kinose Y., White J.R., Press M.F., Drapkin R., Easwaran H., Baylin S.B., Slamon D., Velculescu V.E., Scharpf R.B. Integrated genomic, epigenomic, and expression analyses of ovarian cancer cell lines. Cell Rep. 25:2617-2633(2018)
PubMed=31273215; DOI=10.1038/s41597-019-0116-4 Osorio D., Yu X., Yu P., Serpedin E., Cai J.J. Single-cell RNA sequencing of a European and an African lymphoblastoid cell line. Sci. Data 6:112-112(2019)
PubMed=31401124; DOI=10.1016/j.jmoldx.2019.06.007 Gaedigk A., Turner A., Everts R.E., Scott S.A., Aggarwal P., Broeckel U., McMillin G.A., Melis R., Boone E.C., Pratt V.M., Kalman L.V. Characterization of reference materials for genetic testing of CYP2D6 alleles: a GeT-RM collaborative project. J. Mol. Diagn. 21:1034-1052(2019) |
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