CHO-K1 SV GS双敲除悬浮驯化株
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细胞名称 |
CHO-K1 SV GS双敲除悬浮驯化株 |
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货物编码 |
BFN619481 |
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产品规格 |
T25培养瓶x1 |
1.5ml冻存管x2 |
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细胞数量 |
1x10^6 |
1x10^6 |
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保存温度 |
37℃ |
-198℃ |
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运输方式 |
常温保温运输 |
干冰运输 |
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安全等级 |
1 |
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用途限制 |
仅供科研用途 5类 |
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培养体系 |
DMEM高糖培养基（Hyclone）+10%胎牛血清（Gibco）+1%双抗（Hyclone） |
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培养温度 |
37℃ |
二氧化碳浓度 |
5% |
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简介 |
CHO-K1SV/GS双敲除株取自雌性中国仓鼠，K1SV及GS基因双敲除。此细胞经过悬浮驯化。 |
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注释 |
Group: Serum/protein free medium cell line. |
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STR信息 |
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参考文献 |
PubMed=22068567; DOI=10.1002/bit.24365 Fan L., Kadura I., Krebs L.E., Hatfield C.C., Shaw M.M., Frye C.C. Improving the efficiency of CHO cell line generation using glutamine synthetase gene knockout cells. Biotechnol. Bioeng. 109:1007-1015(2012)
PubMed=25502369; DOI=10.1002/bit.25514 Rajendra Y., Hougland M.D., Alam R., Morehead T.A., Barnard G.C. A high cell density transient transfection system for therapeutic protein expression based on a CHO GS-knockout cell line: process development and product quality assessment. Biotechnol. Bioeng. 112:977-986(2015)
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