正常人肠上皮细胞HIEC-6
BLUEFBIO™ Product Sheet
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细胞名称 |
正常人肠上皮细胞HIEC-6 |
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货物编码 |
BFN60808415 |
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产品规格 |
T25培养瓶x1 |
1.5ml冻存管x2 |
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细胞数量 |
1x10^6 |
1x10^6 |
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保存温度 |
37℃ |
-198℃ |
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运输方式 |
常温保温运输 |
干冰运输 |
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安全等级 |
1 |
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用途限制 |
仅供科研 2类 |
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培养体系 |
DMEM高糖+10%FBS+1%三抗 |
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培养温度 |
37℃ |
二氧化碳浓度 |
5% |
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简介 |
正常人肠上皮细胞HIEC-6取自17-19岁女性，具体年龄不详。上皮样，贴壁培养，可传20代。另该细胞可经SV40转染转变为永生化细胞系。 |
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注释 |
倍增时间96小时。 |
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基因突变 |
/ |
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HLA信息 |
/ |
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STR信息 |
Amelogenin X CSF1PO 11,12 D5S818 11,13 D7S820 8,11 D13S317 11,14 D16S539 12,13 TH01 6,9 TPOX 8,11 vWA 15 |
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参考文献 |
PubMed=8612712; DOI=10.1006/excr.1996.0145 Perreault N., Beaulieu J.-F. Use of the dissociating enzyme thermolysin to generate viable human normal intestinal epithelial cell cultures. Exp. Cell Res. 224:354-364(1996)
PubMed=9827699; DOI=10.1002/(SICI)1097-4644(19981215)71:4<536::AID-JCB8>3.0.CO;2-1 Desloges N., Basora N., Perreault N., Bouatrouss Y., Sheppard D., Beaulieu J.-F. Regulated expression of the integrin alpha9beta1 in the epithelium of the developing human gut and in intestinal cell lines: relation with cell proliferation. J. Cell. Biochem. 71:536-545(1998)
PubMed=20444515; DOI=10.1016/j.ijfoodmicro.2010.03.026 Cencic A., Langerholc T. Functional cell models of the gut and their applications in food microbiology -- a review. Int. J. Food Microbiol. 141:S4-14(2010)
PubMed=28239090; DOI=10.3390/foods1010040 Trapecar M., Cencic A. Application of gut cell models for toxicological and bioactivity studies of functional and novel foods. Foods 1:40-51(2012) |
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