家蚕卵巢细胞BM-N

细胞名称

家蚕卵巢细胞BM-N

货物编码

BFN60806679

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研           3类

培养体系

The base medium for this cell line is IPL-41 Insect Cell Culture Medium with glutamine (available from Sigma or Gibco). To make the complete growth medium, add the following components to the base medium:

2.6 g/L Tryptose Phosphate Broth (TPB)

0.069 mg/L ZnSO4·7H2O

3.86 mg/L AlCl3·6H2O

5 mg/L chlorophenol red

2g/L NaCl

10% heat-inactivated insect grade fetal bovine serum

Adjust pH to 6.3 to 6.4 by adding 1N NaOH

Adjust osmolarity to 317 to 360 by adding extra NaCl

The IPL-41 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation. (In Vitro 17: 495-509, 1981)

培养温度

27℃

二氧化碳浓度

0%

简介

家蚕卵巢细胞BmN取自雌性个体。

序列突变

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注释

Group: Insect cell line.

Group: Recombinant protein production insect cell line.

Characteristics: Infected by Bombyx mori macula-like latent virus (BmMLV).

Omics: Deep proteome analysis.

STR信息

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参考文献

PubMed=8799737; DOI=10.1111/j.1365-2583.1996.tb00053.x

McIntosh A.H., Grasela J.J., Matteri R.L.

Identification of insect cell lines by DNA amplification fingerprinting (DAF).

Insect Mol. Biol. 5:187-195(1996)

PubMed=10777062; DOI=10.1290/1071-2690(2000)036<0205:EOTGFP>2.0.CO;2

Grasela J.J., McIntosh A.H., Goodman C.L., Wilson L.E., King L.A.

Expression of the green fluorescent protein carried by Autographa californica multiple nucleopolyhedrovirus in insect cell lines.

In Vitro Cell. Dev. Biol. Anim. 36:205-210(2000)

PubMed=19496755; DOI=10.1042/BSR20090045

Yao H.-P., Chen L., Xiang X.-W., Guo A.-Q., Lu X.-M., Wu X.-F.

Proteomics identification and annotation of proteins of a cell line of Bombyx mori, BmN cells.

Biosci. Rep. 30:209-215(2010)

PubMed=29133148; DOI=10.1016/j.pep.2017.11.002

Geisler C., Jarvis D.L.

Adventitious viruses in insect cell lines used for recombinant protein expression.

Protein Expr. Purif. 144:25-32(2017)